Rizobia identification promoters of the vegetable growth associated to chickpea (Cicer arietinum L.)


  • Marisel Ortega García Instituto de Investigaciones Fundamentales en Agricultura Tropical “Alejandro de Humboldt” (INIFAT), La Habana, Cuba.
  • Yoania Ríos Rocafull Instituto de Investigaciones Fundamentales en Agricultura Tropical “Alejandro de Humboldt” (INIFAT), La Habana, Cuba.
  • Lily Zelaya Molina Centro Nacional de Recursos Genéticos (CNRG), Tepatitlán de Morelos, Mexico. https://orcid.org/0000-0002-3474-3289
  • Juan Lara Aguilera Centro Nacional de Recursos Genéticos (CNRG), Tepatitlán de Morelos, Mexico.
  • Ramón Orteaga Garibay Centro Nacional de Recursos Genéticos (CNRG), Tepatitlán de Morelos, Mexico.
  • María Caridad Nápoles García Instituto Nacional de Ciencias Agrícolas, Mayabeque, Cuba. https://orcid.org/0000-0003-1413-1717




taxonomy, characterization, nodulation, selection, yield


Introduction. Rhizobium has been used as a growth stimulator in leguminous. Different species of this group are associated with chickpea (Cicer arietium L.), so it is important to obtain isolated with capacity of benefitting their growth and productivity. Objective. To select and identify isolates of Rhizobium sp. in terms of their attributes as plant growth-promoting rhizobacteria with greater effectiveness in their association with chickpea cultivars. Materials and methods. The studies were carried out during the years 2017-2020, at the Instituto de Investigaciones Fundamentales en Agricultura Tropical “Alejandro of Humboldt”, Havana, Cuba. Three rhizobia strains, obtained from chickpea nodules were identified by 16S RNA gene sequencing and characterized for their potential as growth-promoting bacteria. An experiment was carried out in field conditions in two campaigns to demonstrate their effectiveness in the interaction with plants. For the experiments under laboratory conditions a completely randomized design was used, while the field trials were conducted under a randomized block design, in both cases an analysis of variance was performed. Results. The three bacterial strains associated with chickpea and identified as Rhizobium sp. fixed atmospheric nitrogen, solubilized calcium phosphate, released phytohormones and were able to inhibit the growth of Fusarium oxysporumFusarium incarnatum, and Fusarium moniliforme. Inoculation in chickpea increased the nodulation and the crop yield variables such as number of pods per plant and fresh grain mass (g). Conclusions. Rhizobium sp. strains were identified with attributes as plant growth-promoting bacteria associated with national chickpea cultivars, that increased their nodulation and yield.


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How to Cite

Ortega García, M., Ríos Rocafull, Y., Zelaya Molina, L., Lara Aguilera, J., Orteaga Garibay, R., & Nápoles García, M. C. . (2023). Rizobia identification promoters of the vegetable growth associated to chickpea (Cicer arietinum L.). Agronomía Mesoamericana, 34(2), 50929. https://doi.org/10.15517/am.v34i2.50929

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