Endophytic fungi associated to Vitis vinifera with antibiosis capacity against Botrytis cinerea
DOI:
https://doi.org/10.15517/am.2024.56193Keywords:
antagonism, metabolites, mold, biocontrolAbstract
Introduction. Endophytic fungi (EF) in grapevines (Vitis vinifera) hold significant potential for agricultural applications and could serve as potential biological control agents for vineyards. Objective. To isolate and identify endophytic fungi (EF) from grapevine plants capable of displaying antibiosis towards Botrytis cinerea. Materials and methods. Young and mature branches were collected from forty healthy grapevine plants across seven production lots in the Cañete province, Lima, Peru, between March and May 2022. EF isolation was carried out from 702 disinfected tissue sections on potato dextrose agar (PDA) medium, with genus-level identification. Secondary metabolites were extracted from twenty-eight EF strains to determine their inhibition of the pathogen’s mycelium growth. Analysis of variance (ANOVA) was conducted using the Minitab statistical software for evaluated parameters. Results. A total of 129 EF strains were isolated from leaves and stems of young and mature grapevine branches, encompassing seven genera (Trichoderma, Aspergillus, Alternaria, Cladosporium, Fusarium, Nigrospora, and Stemphylium). The highest frequency occurred in leaves and mature branches; Trichoderma and Alternaria were the most prevalent genera, constituting 22.48% and 21.71% of total isolates, respectively. Metabolites derived from the EFVH-01 strain of the Cladosporium genus, as well as EF-99 and EF-70 strains of the Alternaria genus, displayed the greatest impact on inhibiting the pathogen’s mycelium growth. Conclusions. Strains of endophytic fungi of the genus Cladosporium and Alternaria showed antibiosis capacity towards Botrytis cinerea at the laboratory scale and could potentially serve as biocontrols, offering an alternative to chemical disease management in grapevines.
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Copyright (c) 2024 Betsabe León-Ttacca, Juan Alca-Zavala, Jefferson Rosas-Martínez, Eddy Robles-Perez, Paola Zamudio-Eustaquio, Luis García-Díaz
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