Zinc oxide supplementation as a substitute to antibiotics in pigs (Sus scrofa domestica)

Authors

DOI:

https://doi.org/10.15517/am.2024.54643

Keywords:

hemoglobyn, leukocytes, body weight, diarrhea

Abstract

Introduction. There is a high human concern about resistance to antibiotics due to their inappropriate use as growth promoters in animal husbandry. Objective. To evaluate the effect of a high level of zinc as a substitute to antibiotics in nursery pigs. Material and methods. The study was carried out from June to August 2022 in the province of Chiriqui, Panama. The experimental period consisted of three phases with 14 days each. The nursery pigs (n= 54) were distributed to one of three treatments with six replicates per treatment and three pigs per replicate. The treatments were: 1) control diet, which was formulated to meet the nutritional requirements for nursery pigs; 2) similar to treatment 1, plus 200 mg of amoxicillin /kg of feed and 40 mg of florfenicol /kg of feed during phase 1 (P1) and phase 2 (P2), respectively; and 3) similar to treatment 1, plus 2000 ppm and 1600 ppm of zinc during P1 and P2, respectively. In phase 3 (P3), all pigs were fed a common diet. Results. The supplementation of zinc improved the average daily gain in all phases. Pigs fed zinc diet had better F:G ratio in P2 compared to pigs fed antibiotic diet (p<0.05). A higher concentration of hemoglobin and percentage of monocytes was found in pigs fed with higher level of zinc than the other treatments. (p<0.05). Furthermore, Pigs fed the antibiotic, or the control diet had a lower percentage of fecal dry matter compared to those supplemented with zinc. (p<0.05). Conclusion. The supplementation of high levels of zinc exerted improvements, in comparison to the antibiotic, in terms of hemoglobin concentration, incidence of diarrhea and weight gain in pigs during 42 days after weaning.

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Author Biography

Reggie Guerra , Universidad de Panamá/Sistema Nacional de Investigación, Chiriquí, Panamá

Facultad de Ciencias Agropecuarias

Departamento de Zootecnia

Profesor

References

Association of Analytical Chemists. (2016). Official Methods of Analysis (20th Ed.). AOAC International.

Broxmeyer, H. E. (2013). Erythropoietin: multiple targets, actions, and modifying influences for biological and clinical consideration. Journal of Experimental Medicine, 210(2), 205–208. https://doi.org/10.1084/jem.20122760

Boudry, G., Péron, V., Le Huërou-Luron, I., Lallès, J. P., & Sève, B. (2004). Weaning induces both transient and long-lasting modifications of absorptive, secretory, and barrier properties of piglet intestine. Journal of Nutrition, 134(9), 2256–2262. https://doi.org/10.1093/jn/134.9.2256

Campbell, J. M., Crenshaw, J. D., & Polo, J. (2013). The biological stress of early weaned piglets. Journal of Animal Science and Biotechnology, 4, Article 19. https://doi.org/10.1186/2049-1891-4-19

Chen, Y. H., Feng, H. L., & Jeng, S. S. (2018). Zinc supplementation stimulates red blood cell formation in rats. International Journal of Molecular Science, 19(9), Article 2824. https://doi.org/10.3390/ijms19092824

de Lange, C. F., Pluske, J., Gong, J., & Nyachoti, C. M. (2010). Strategic use of feed ingredients and feed additives to stimulate gut health and development in young pigs. Livestock Science, 134(1–3), 124–134. https://doi.org/10.1016/j.livsci.2010.06.117

Feng, H. L., Chen, Y. H., & Jeng, S. S. (2019). Effect of zinc supplementation on renal anemia in 5/6-nephrectomized rats and a comparison with treatment with recombinant human erythropoietin. International Journal of Molecular Science, 20(20), Article 4985. https://doi.org/10.3390/ijms20204985

Gresse, R., Chaucheyras, F., Fleury, M. A., Van de Wiele, T., Forano, E., & Blanquet, S. (2017). Gut Microbiota Dysbiosis in Postweaning Piglets: Understanding the Keys to Health. Trends in Microbiology, 25(10), 851–873. https://doi.org/10.1016/j.tim.2017.05.004

Goering, M., & Van Soest, P. (1970). Forage fiber analysis: apparatus, reagents, procedures and some applications. United States Deparment of Agriculture. https://naldc.nal.usda.gov/download/CAT87209099/PDF

Grondin, J. A., Kwon, Y. H., Far, P. M., Haq, S., & Khan, W. I. (2020). Mucins in intestinal mucosal defense and inflammation: learning from clinical and experimental studies. Frontiers in Immunology, 11, Article 2054. https://doi.org/10.3389/fimmu.2020.02054

Haase, H., & Rink, L. (2009). The immune system and the impact of zinc during aging. Immunity and Ageing, 6, Article 9. https://doi.org/10.1186/1742-4933-6-9

Han, Y. K., Hwan, I. L., & Thacker, P. A. (2011). Use of a micro-encapsulated eucalyptus-medium chain fatty acid product as an alternative to zinc oxide and antibiotics for weaned pigs. Journal of Swine Health and Production, 19(1), 34–43.

He, Y., Yuan, Q., Mathieu, J., Stadler, L., Senehi, N., Sun, R., & Alvarez, P. J. (2020). Antibiotic resistance genes from livestock waste: occurrence, dissemination, and treatment. Npj Clean Water, 3(1), 1–11. https://doi.org/10.1038/s41545-020-0051-0

Hedemann, M. S., Jensen, B. B., & Poulsen, H. D. (2006). Influence of dietary zinc and copper on digestive enzyme activity and intestinal morphology in weaned pigs. Journal of Animal Science, 84(12), 3310–3320. https://doi.org/10.2527/jas.2005-701

Hill, G. M., Mahan, D. C., Carter, S. D., Cromwell, G. L., Ewan, R. C., Harrold, R. L., Lewis, A. J., Miller, P. S., Shurson, G. C., & Veurn, T. L. (2001). Effect of pharmacological concentrations of zinc oxide with or without the inclusion of an antibacterial agent of nursery pig performance. Journal of Animal Science, 79(4), 934-941. https://doi.org/ 10.2527/2001.794934x

Huber, K. L., & Cousins, R. J. (1993). Zinc metabolism and metallothionein expression in bone marrow during erythropoiesis. American Journal of Physiology, Endocrinology and Metabolism, 264(5), E770–E775. https://doi.org/10.1152/ajpendo.1993.264.5.E770

Jeng, S. S., & Chen, Y. H. (2022). Association of zinc with anemia. Nutrients, 14(22), Article 4918. https://doi.org/10.3390/nu14224918

Kim, J. C., Mullan, B. P., Hampson, D. J., & Pluske, J. R. (2007). Addition of oat hulls to an extruded rice-based diet for weaner pigs ameliorates the incidence of diarrhoea and reduces indices of protein fermentation in the gastrointestinal tract. British Journal of Nutrition, 99(6), 1217–1225. https://doi.org/10.1017/S0007114507868462

Kim, S. jae, C,. Park, B. C., Lee, C. Y., & Han, J. H. (2015). Effects of a lipid-encapsulated zinc oxide dietary supplement, on growth parameters and intestinal morphology in weanling pigs artificially infected with enterotoxigenic Escherichia coli. Journal of Animal Science and Technology, 57(1), 1–5. https://doi.org/10.1186/s40781-014-0038-9

Knight, L. C., & Dilger, R. N. (2018). Longitudinal effects of Iron deficiency anemia and subsequent repletion on blood parameters and the rate and composition of growth in pigs. Nutrients, 10, Article 5. https://doi.org/10.3390/nu10050632

Konomi, A., & Yokoi, K. (2005). Zinc deficiency decreases plasma erythropoietin concentration in rats. Biological Trace Elements Research, 107(3), 289–292. https://doi.org/10.1385/BTER:107:3:289

Lee, S. H., Shinde, P., Choi, J., Park, M., Ohh, S., Kwon, I. K., Pak, S. I., & Chae, B. J. (2008). Effects of dietary iron levels on growth performance, hematological status, liver mineral concentration, fecal microflora, and diarrhea incidence in weanling pigs. Biological Trace Element Research, 126, 57–68. https://doi.org/10.1007/s12011-008-8209-5

Lei, X. J., & Kim, I. H. (2018). Low dose of coated zinc oxide is as effective as pharmacological zinc oxide in promoting growth performance, reducing fecal scores, and improving nutrient digestibility and intestinal morphology in weaned pigs. Animal Feed Science and Technology, 245, 117–125. https://doi.org/10.1016/j.anifeedsci.2018.06.011

Martin, S. J., Mazdai, G., Strain, J. J., Cotter, T. G., & Hannigan, B. M. (1991). Programmed cell death (apoptosis) in lymphoid and myeloid cell lines during zinc deficiency. Clinical & Experimental Immunology, 83(2), 338–343. https://doi.org/10.1111/j.1365-2249.1991.tb05639.x

Mudarra, R., Norato, J., Guerra, R., & Melgar, A. (2022). Effects of Supplementing pharmacological levels of zinc oxide on productive performance, hematological profile and diarrhea control in pigs. Revista Investigaciones Agropecuarias, 4(2), 58–72.

National Research Council. (2012). Nutritional requirements of swine (11th ed.). The National Academies Press.

Oh, H., Park, Y., Cho, J. H., Song, M., Gu, B., Yun, W., Lee, J., An, J., Kim, Y., Lee, J., Kim, S., Kim, H., Kim, E. S., Lee, B., Kim, B., Kim, H. B., Cho, J., & Kim, M. (2021). Changes in diarrhea score, nutrient digestibility, zinc utilization, intestinal immune profiles, and fecal microbiome in weaned piglets by different forms of zinc. Animals, 11, Article 5. https://doi.org/10.3390/ani11051356

Pieper, R., Vahjen, W., Neumann, K., Van Kessel, A. G., & Zentek, J. (2012). Dose-dependent effects of dietary zinc oxide on bacterial communities and metabolic profiles in the ileum of weaned pigs. Journal of Animal Physiology and Animal Nutrition, 96(5), 825–833. https://doi.org/10.1111/j.1439-0396.2011.01231.x

Sapkota, M., & Knoell, D. L. (2018). Essential role of zinc and zinc transporters in myeloid cell function and host defense against infection. Journal of Immunology Research, 2018, Article 4315140. https://doi.org/10.1155/2018/4315140

Seip, V., Friendship, R., Amezcua, R., & Farzan, A. (2020). The relationship between hemoglobin levels at weaning and growth performance and antibody response in nursery pigs. Canadian Veterinary Journal, 61(11), 1170–1174.

Shin, S. J., Kang, S. G., Nabin, R., Kang, M. L., & Yoo, H. S. (2005). Evaluation of the antimicrobial activity of florfenicol against bacteria isolated from bovine and porcine respiratory disease. Veterinary Microbiology, 106(1–2), 73–77. https://doi.org/10.1016/j.vetmic.2004.11.015

Verstegen, M. W. A., & Williams, B. A. (2002). Alternatives to the use of antibiotics as growth promoters for monogastric animals. Animal Biotechnology, 13(1), 113–127. https://doi.org/10.1081/ABIO-120005774

Wang, Q., Ying, J., Zou, P., Zhou, Y., Wang, B., Yu, D., Li, W., & Zhan, X. (2020). Sodium and zinc oxide on growth performance, immune status and antioxidant capacity of weaned piglets. Animals, 10, Article 11. https://doi.org/10.3390/ani10112104

Wei, X., Tsai, T., Knapp, J., Bottoms, K., Deng, F., Story, R., Maxwell, C., & Zhao, J. (2020). ZnO modulates swine gut microbiota and improves growth performance of nursery pigs when combined with peptide cocktail. Microorganisms, 8, Article 2. https://doi.org/10.3390/microorganisms8020146

Wensley, M. R., Tokach, M. D., Woodworth, J. C., Goodband, R. D., Gebhardt, J. T., DeRouchey, J. M., & McKilligan, D. (2021). Maintaining continuity of nutrient intake after weaning. II. Review of post-weaning strategies. Translational Animal Science, 5(1), 1–16. https://doi.org/10.1093/tas/txab022

Wijtten, P, Meulen, J. Van Der, & Verstegen, M. (2011). Intestinal barrier function and absorption in pigs after weaning: A review. British Journal of Nutrition, 105(7), 967–981. https://doi.org/10.1017/S0007114510005660

Yu, T., Zhu, C., Chen, S., Gao, L., Lv, H., Feng, R., Zhu, Q., Xu, J., Chen, Z., & Jiang, Z. (2017). Dietary high zinc oxide modulates the microbiome of ileum and colon in weaned piglets. Frontiers in Microbiology, 8, Article 825. https://doi.org/10.3389/fmicb.2017.00825

Yun, J., Olkkola, S., Hänninen, M. L., Oliviero, C., & Heinonen, M. (2017). The effects of amoxicillin treatment of newborn piglets on the prevalence of hernias and abscesses, growth and ampicillin resistance of intestinal coliform bacteria in weaned pigs. Plos One, 12, Article 2. https://doi.org/10.1371/journal.pone.0172150

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Published

2024-01-09

How to Cite

Barría, C., Mudarra, R. ., & Guerra , R. . (2024). Zinc oxide supplementation as a substitute to antibiotics in pigs (Sus scrofa domestica). Agronomía Mesoamericana, 54643. https://doi.org/10.15517/am.2024.54643

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