Revista de Biología Tropical ISSN Impreso: 0034-7744 ISSN electrónico: 2215-2075

OAI: https://www.revistas.ucr.ac.cr/index.php/rbt/oai
In vitro antioxidant activity and qualitative phytochemical analysis of two Vismia (Hypericaceae) species collected in Los Andes, Venezuela
PT 64-4 dic 2016
HTML
PDF

Keywords

antioxidant activity
DPPH
total phenolic content
total flavonoids content
qualitative phytochemical analysis
Vismia baccifera
Vismia macrophylla.
actividad antioxidante
DPPH
fenoles totales
contenido de flavonoides
análisis fitoquímico cualitativo
Vismia baccifera
Vismia macrophylla.

How to Cite

Buitrago D., A., Rojas-Vera, J., & Peñaloza, Y. (2016). In vitro antioxidant activity and qualitative phytochemical analysis of two Vismia (Hypericaceae) species collected in Los Andes, Venezuela. Revista De Biología Tropical, 64(4), 1431–1439. https://doi.org/10.15517/rbt.v64i4.22968

Abstract

Vismia genus is distributed mainly in tropical and subtropical regions of Central, South America and some areas of Africa. According to previous investigations, antioxidant potential of Vismia species might be related to anthrones, anthraquinones, flavonoids and phenol derivatives biosynthesized by these plants. In this investigation, phytochemical screening of Vismia baccifera (VB) from Mérida-Venezuela and Vismia macrophylla (VM) from Táchira-Venezuela methanolic extracts, carried out using various chemical assays, revealed an abundant presence of anthraquinones in both species analyzed. Glycosides were also present while flavones and dehydroflavones were observed abundantly in VB but moderated in VM. Triterpenes were also detected and steroids showed to be abundant in VM but moderate in VB. On the other hand, antioxidant capacity measured by the DPPH assay showed that VM possesses a stronger antioxidant activity than VB with IC50 5.50 µg mL-1. Phenol and flavonoid assays carried out by Folin-Ciocalteu and colorimetric test also revealed that methanol extracts of both species contain high concentrations of these metabolites. A relationship between the antioxidant activity, total phenol and flavonoids content of the extracts analyzed was demonstrated in this investigation since those samples with higher phenolic concentrations showed likewise higher antioxidant activity.

https://doi.org/10.15517/rbt.v64i4.22968
HTML
PDF

References

Álvarez, E., Jiménez, O., Posada, C., Rojano, B., Gil, J., García, C., & Durango, D. (2008). Actividad antioxidante y contenido fenólico de los extractos provenientes de las bayas de dos especies del género Vismia (Guttiferae). VITAE, 15, 165-172.

Baydar, N. G., Özkan, G., & Yasar, S. (2007). Evaluation of the antiradical and antioxidant potential of grape extracts. Food Control, 18, 1131-1136.

Botta, B., Delle-Monache, G., Delle-Monache, F., Marini-Bettolo, G. B., & Menichini, F. (1986). Vismione H and prenylated xanthones from Vismia guineensis. Phytochemistry, 25, 1217-1219.

Brands-Williams, W., Cuvelier, M. E., & Berset, C. (1995). Use of a free radical method to evaluated antioxidant activity. LWT- Food Science and Technology, 28, 25-30.

Fuller, R., Westergaard, C., Collins, J., Cardellina, J., & Boyd, M. (1999). Vismiaphenones D-G from Vismia cayennensis. Journal of Natural Products, 62, 67-69.

Goupy, P., Bautista, A., Fulcrand, H., & Dangles, O. (2009). Antioxidant activity of wine pigments derived from anthocyanins: Hydrogen transfer reactions to the DPPH radical and inhibition of the heme-induced peroxidation of linoleic acid. Journal of Agricultural and Food Chemistry, 57, 5762-5770.

Gutiérrez, M., A., Pino, B. N., & Cuesta, L. J. A. (2011). Actividad antioxidante y contenido total de fenoles de varios extractos etanólicos de plantas medicinales. Investigación, Biodiversidad y Desarrollo, 30(1), 66-73.

Hossain, M. A., & Shah, M. D. (2015). A study on the total phenols content and antioxidant activity of essential oil and different solvent extracts of endemic plant Merremia borneensis. Arabian Journal of Chemistry, 8, 66-71.

Hussain, H., Hussain, J., Al-Harrasi, A., Saleem, M., Green, I. R., van Ree, T., & Ghulam, A. (2012). Chemistry and biology of genus Vismia. Pharmaceutical Biology, 50, 1448-1462.

Kim, D. O., Chun, O. K., Kim, Y. J., Moon, H. Y., & Lee, C. (2003). Quantification of phenolics and their antioxidant capacity in fresh plums. Journal of Agricultural and Food Chemistry, 51, 6509-6515.

Lizcano, L. J., Siles, M., Trepiana, J., Hernández, L., Navarro, R., Ruiz-Larrea, B., & Ruiz-Sanz, J. I. (2015). Piper and Vismia species from Colombian Amazonia differentially affect cell proliferation of hepatocarcinoma cells. Nutrients, 7, 179-195.

Lizcano, L. J., Fadil-Bakkali, M., Ruiz-Larrea, B., & Ruiz-Sanz, J. I. (2010). Antioxidant activity and polyphenol content of aqueous extracts from Colombian Amazonian plants with medicinal use. Food Chemistry, 119(4), 1566-1570.

Nagem, T., & de Oliveira, F. (1997). Xanthones and other constituents of Vismia parviflora. Journal of the Brazilian Chemical Society, 8, 505-508.

Parajuli, S., Pun, N. T., Parajuli, Sh., & Jamarkattel-Pandit, N. (2012). Antioxidant activity, total phenol and flavonoid contents in some selected medicinal plants of Nepal. JHAS, 2, 27-31.

R Core Team. (2015). R: A language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria. Retrieved from http://www.R-project.org/.

Raghavendra, M., Madhusudhana, A., Raghuveer, P., Sudharshan, A., & Siva, L. (2013). Comparative studies on the in vitro antioxidant properties of methanolic leafy extracts from six edible leafy vegetables of India. Asian Journal of Pharmaceutical and Clinical Research, 6, 96-99.

Ribeiro, A. B., Berto, A., Ribeiro, D., Freitas, M., Chisté, R. C., Visentainer, J. V., & Fernandes, E. (2015). Stem bark and flower extracts of Vismia cauliflora are highly effective antioxidants to human blood cells by preventing oxidative burst in neutrophils and oxidative damage in erythrocytes. Pharmaceutical Biology, 53(11), 1691-1698.

Sarabjot, K., & Poonam, M. (2014). Study of total phenolic and flavonoid content, antioxidant activity and antimicrobial properties of medicinal plants. Journal of Microbiology & Experimentation, 1, 1-6.

Shah, M. D., & Hossain, M. A. (2014). Total flavonoids content and biochemical screening of the leaves of tropical endemic medicinal plant Merremia borneensis. Arabian Journal of Chemistry, 7, 1034-1038.

Shyamala-Gowri, S., & Vasantha, K. (2010). Phytochemical screening and antibacterial activity of Syzygium cumini (L.) (Myrtaceae) leaves extracts. International Journal of PharmTech Research, 2, 1569-1573.

Škerget, M., Kotnik, P., Hadolin, M., Hraš, A. R., Simonič, M., & Knez, Ž. (2005). Phenols, proanthocyanidins, flavones and flavonols in some plant materials and their antioxidant activities. Food Chemistry, 89, 191-198.

Tepe, B., & Sokmen, A. (2007). Screening of the antioxidative properties and total phenolic contents of three endemic Tanacetum subspecies from Turkish flora. Bioresource Technology, 98, 3076-3079.

Tiwari, P., Kumar, B., Kaur, M., Kaur, G., & Kaur, H. (2011). Phytochemical screening and extraction: A Review. Internationale Pharmaceutica Sciencia, 1, 98-106.

Trease, G. E., & Evans, W. C. (2002). Pharmacognosy. England: Ed. Saunders Publishers.

Vizcaya, M., Morales, A., Rojas, J., & Nuñez, R. (2012). Revisión bibliográfica sobre la composición química y actividades farmacológicas del género Vismia (Guttiferae). Boletín Latinoamericano y del Caribe de Plantas Medicinales y Aromáticas, 11(1), 12-34.

Comments

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Copyright (c) 2016 Revista de Biología Tropical

Downloads

Download data is not yet available.