Revista de Biología Tropical ISSN Impreso: 0034-7744 ISSN electrónico: 2215-2075

OAI: https://www.revistas.ucr.ac.cr/index.php/rbt/oai
Acute lethal effect of the commercial formulation of the insecticides Imidacloprid, Spinosad y Thiocyclam hidrogenoxalate in Bombus atratus (Hymenoptera: Apidae) workers
PT 64-4 dic 2016
PDF (Español (España))
HTML (Español (España))

Supplementary Files

Untitled (Español (España))

Keywords

Dosis letal media (DL50)
toxicidad
polinizadores
abejorros.
Bumblebees
lethal median dose
DL50
pollinators
toxicity.

How to Cite

Riaño Jiménez, D., & Cure, J. R. (2016). Acute lethal effect of the commercial formulation of the insecticides Imidacloprid, Spinosad y Thiocyclam hidrogenoxalate in Bombus atratus (Hymenoptera: Apidae) workers. Revista De Biología Tropical, 64(4), 1737–1745. https://doi.org/10.15517/rbt.v64i4.21521

Abstract

The effect of insecticides on bees has gained great attention, however, there are few studies that explore this issue on Neotropical bees. Bombus atratus is a neotropical species broadly distributed in Colombia and is considered an important pollinator of both Andean ecosystems and agroecosystems. However, as for many wild bees species, the effect of insecticides on B. atratus is unknow. In this study we determined the acute median lethal dose (LD50) of commercial formulations of insecticides Imidacloprid, Spinosad and Thiocyclam hydrogen oxalate, widely used in Colombia to control several pests of important crops. The LD50 was carried out by oral and contact routes, following and modifying the EPPO and OECD guidelines to perform LD50 on A. mellifera. We evaluated five doses for each route and insecticide, in a total of 25 medium-size workers for each dose by duplicate. Mortality was registered at 24, 48 and 72 hours after the experiment; and data were analyzed with the Probit regression model. For Imidacloprid, contacts and oral LD50 were 0.048 µg/bee and 0.010 µg/bee, respectively. For Thiocyclam hydrogen oxalate, topical and oral LD50 were 0.244 µg/bee and 0.056 µg/bee, respectively. For Spinosad, the oral LD50 corresponded to 0.28 µg/bee; it was not possible to establish the LD50 for the contact route. The Hazard Quotient (HQ) and Index of Relative Toxicity indicated that all three active ingredients are highly toxic. We discussed the risk of the insecticides use on B. atratus, considering their chemical nature.
https://doi.org/10.15517/rbt.v64i4.21521
PDF (Español (España))
HTML (Español (España))

References

Abrahamovich, A., Diaz, N., & Morrone, J. (2004). Distributional patterns of the Neotropical and Andean species of the genus Bombus (Hymenoptera: Apidae). Acta Zoológica Mexicana, 20(1), 99-117.

Aldana, J., Cure, J. R., Almanza, M. T., Vecil, D., & Rodríguez, D. (2007). Efecto de Bombus atratus (HYMENOPTERA: APIDAE) sobre la productividad de tomate (Lycopersicon esculentum Mill) bajo invernadero en la Sabana de Bogotá, Colombia. Agronomía Colombiana, 25(1), 62-72.

Almanza, M. (2007). Management of Bombus atratus bumblebees to pollinate lulo (Solanum quiotense L), a native fruit from the Andes of Colombia. ZEF. Ecology and Development Series, 50.

Belloti, A. C., Cardona, C., & Lapoite, S. L. (1990). Trends in pesticide use in Colombia and Brazil. Journal of Agricultural Entomology, 7(3), 191-201.

Besard, L., Mommaerts, V., Abdu-alla, G., & Smagghe, G. (2011) Lethal and sublethal side effects assessment supports a more bening profile of spinetoram compared with spinosad in bumble bee Bombus terrestris. Pest Management Science, 67(5), 541-547.

Biesmeijer, J. C., Roberts, P. M., Reemer, M., Ohlemüller, R., Edwards, M., Peeters, T., Schaffers, A. P., Potts, S. G., Kleukers, R., Thomas, C., D., Settele, J., & Kunin, W. E. (2006). Parallel declines in pollinators and insect-pollinated plants in Britain and Netherlands. Science, 313, 351-354.

Biondi, A., Mommaerts, V., Smagghe, G., Viñuela, E., Zappala, L., & Desneux, N. (2012). The non-target impact of spinosyns on benefical arthropods. Pest Management Science, 68, 1523-1536

Blacquière, T., Smagghe, G., van Gestel, C., & Mommaerts, V. (2012). Neonicotinoids in bees: a review of concentrations, side effects and risk assessment. Ecotoxicology, 21, 973-992.

Bojaca, C. R., Gil, R., Casilimas, H., & Schrevens, E. (2012). Modelling the environmental impact of pesticides sprayed on greenhouses tomatoes. A regional case study in Colombia. Acta Horticulturae, 957, 61-68.

Colla, S., & Packer, L. (2008). Evidence for decline in eastern North American bumble bees (Hymenoptera: Apidae), with special focus on Bombus affinis Creason. Biodiversity and Conservation, 17, 1379-1391.

Cruz, P., Almanza, M. T., & Cure, J. R. (2007). Logros y perspectivas de la cría de abejorros del género Bombus en Colombia. Revista de la Facultad de Ciencias Básicas y Aplicadas, 3(1), 49-60.

Dar, S., Khan, Z., & Abass, A. (2010). Pollinator decline: a major issue in crop production. Research Journal of Agricultural Sciences, 1(4), 491-493.

Desneux, N., Decourtye, A., & Delpuech, J. M. (2007). The subletal effects of pesticides on beneficial arthropods. Annual Review of Entomology, 52, 81-106.

Freitas, B., Imperatriz-Fonseca, V. L., Medina, L. M., Kleinert, A. M., Galetto, L., Nates-Parra, G., & Quezada-Euán, J. (2009). Diversity threats and conservation of native bees in the tropics. Apidologie, 40, 332-346.

Gómez, H. (1999). Algunos métodos estadísticos para el estudio de poblaciones de organismos. Colombia: Facultad de Ciencias Agrícolas, Universidad Nacional de Colombia.

Gordon, F., Rizzardi, M., Vinson, S. B., & Griswold, T. (2009). Decline in bee diversity and abundance from 1972-2004 on a flowering leguminous tree, Andira inermis in Costa Rica at the interface of disturbed dry forest and the urban environment. Journal of the Kansas Entomolgical Society, 82(1), 1-20.

Greenleaf, S., & Kremen, C. (2006). Wild bee species increase tomato production and respond differently to surrounding land use in northern California. Biological Conservation, 133, 81-87.

Klein, A. M., Vaissiere, B. E., Cane, J., Steffan-Dewenter, I., Cunningham, S., Kremen, C., & Tscharntke, T. (2009). Importance of pollinators in changing landscapes for world crops. Proceedings of the Royal Society of London, 274, 303-313.

Marletto, F., Patetta, A., & Manino, A. (2003). Laboratory assessment of pesticide toxicity to bumblebees. Bulletin of Insectology, 56(1): 155-158.

Marrs, T., & Ballantyne, B. (2004). Pesticide toxicology and international regulation. England: John Willey & Sons, Ltd.

Mayes, M. A., Thompson, G. D., Husband, B., & Miles, M. M. (2003). Spinosad toxicity to pollinators and associated risk. Reviews of Environmental Contamination and Toxicology, 179, 37-71

Mineau, P., Harding, K. M., Whiteside, M., Fletcher, M. R., Garthwaite, D., & Knopper, I. D. (2008). Using reports of bee mortality in the field to calibrate laboratory-derived test pesticide risk indices. Environmental Entomology, 3(2), 546-554.

Morandin, L., Winston, M. L., Franklin, M. T., & Abbott, V. A. (2005). Lethal and sub-lethal effects of spinosad on bumble bee (Bombus impatiens Cresson). Pest Management Science, 61, 619-625.

Nates-Parra, G., & González, V. H. (2000). Las abejas silvestres de Colombia: Por qué y cómo conservarlas. Acta Biologica Colombiana, 5(1), 5-37.

OECD. (1998a). Guidelines for the testing of chemicals number 213. Honeybees, acute oral toxicity test. OECD. Paris: Environmental health and safety division.

OECD. (1998b). Guidelines for the testing of chemicals number 213, Honeybees, acute contact toxicity test. OECD. Paris: Environmental health and safety division.

Ospina, R., Lievano, A., & Nates-Parra, G. (1987). Patrón de Coloración del Abejorro Social Bombus atratus, Franklin en Cundinamarca, Colombia. Una Población Diferenciada. Revista de Biología Tropical, 35(2), 317-324.

Pacateque, J., Cruz, P., Aguilar, M., & Cure, J. R. (2012). Efecto de la alimentación vía bolsillo en etapas tempranas de desarrollo de Bombus atratus (Hymenoptera: Apidae). Revista Colombiana de Entomología, 38(2), 343-346.

Pinheiro, J., & Freitas, B. (2010). Efeitos letais dos pesticidas agrícolas sobre polinizadores e perspectivas de manejo para os agroecossistemas brasileiros. Oecologia Asutralis, 14(1), 266-281.

Potts, S. G., Biesmeijer, J. C., Kremen, C., Neumann, P., Schweiger, O., & Kunin, W. (2010). Global pollinator declines trends impacts and drivers. Trends in Ecology & Evolution, 25(6), 345-353.

Riaño, D., Veloza, M., Cure, J. R., & Almanza, M. T. (2014). Desarrollo de dos colonias de Bombus atratus (Hymenoptera: Apidae) mantenidas bajo dos modos de alimentación. Revista de la Facultad de Ciencias Básicas y Aplicadas, 10(2), 132-141.

Roberts, T., & Hutson D. (1999). Metabolic pathways of agrochemicals. Part two. The Royal Society of Chemistry.

Robertson, J. L., Russell, R. M., Preisler, H. K., & Savin, N. E. (2007). Bioassays with arthropods. CRS press, Taylor & Francis Group.

Rundlöf, M., Nilsson, H., & Smith, H. (2008). Interacting effects of farming practice and landscape context on bublebees. Biological Conservation, 141, 417-426.

Sánchez-Bayo, F. (2012). Insecticides Mode of Action in Relation to Their Toxicity to Non-Target Organisms. Journal of Environment Analytic Toxicology, S4:002 doi:10.4172/2161-0525.S4-002

Scott-Dupree, C. D., Conroy, L., & Harris, C. R. (2009). Impact of currently used or potentially useful insecticides for canola agroecosystems on Bombus impatiens (Hymenoptera: Apidae), Megachile rotundata (Hymenoptera: Megachilidae) and Osmia lignaria (Hymenoptera: Megachilidae). Journal of Economic Entomology, 102(1), 177-182.

van der Steen, J. (2001). Review of the methods to determine the hazard and toxicity of pesticides on bumblebees. Apidologie, 32, 399-406.

Thompson, H., & Hunt, L. (1999). Extrapolating from honey bees to bumblebees in pesticide risk assessment. Ecotoxicology, 8(3), 147-166.

Thompson, H. (2001). Assessing the exposure and toxicity of pesticides to bumblebees (Bombus spp). Apidologie, 32, 305-321.

USDA. (2009). Technical Group Report on Honeybee Toxicity Testing. July 8-9.

Comments

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Copyright (c) 2016 Revista de Biología Tropical

Downloads

Download data is not yet available.