Revista de Biología Tropical ISSN Impreso: 0034-7744 ISSN electrónico: 2215-2075

OAI: https://www.revistas.ucr.ac.cr/index.php/rbt/oai
Aspergillus and Penicillium (Eurotiales: Trichocomaceae) in soils of the Brazilian tropical dry forest: diversity in an area of environmental preservation
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Keywords

Catimbau
Caatinga
fungi
richness
taxonomy
Catimbau
Caatinga
hongos
riqueza
taxonomía.

How to Cite

do Nascimento Barbosa, R., Pereira Bezerra, J. D., Oller Costa, P. M., Correia de Lima-Júnior, N., Gomes Alves de Souza Galvão, I. R., dos Santos-Júnior, A. A., Fernandes, M. J., Souza-Motta, C. M., & Oliveira, N. T. (2016). Aspergillus and Penicillium (Eurotiales: Trichocomaceae) in soils of the Brazilian tropical dry forest: diversity in an area of environmental preservation. Revista De Biología Tropical, 64(1), 45–53. https://doi.org/10.15517/rbt.v64i1.18223

Abstract

Soil is a complex biological system that plays a key role for plants and animals, especially in dry forests such as the Caatinga. Fungi from soils, such as Aspergillus and Penicillium, can be used as bioindicators for biodiversity conservation. The aim of this study was to isolate and identify species of Aspergillus and Penicillium in soil, from the municipalities of Tupanatinga and Ibimirim, with dry forests, in the Catimbau National Park. Five collections were performed in each area during the drought season of 2012, totaling 25 soil samples per area. Fungi were isolated by suspending soil samples in sterile distilled water and plating on Sabouraud Agar media plus Chloramphenicol and Rose Bengal, and Glycerol Dicloran Agar. Isolates were identified by morphological taxonomy in the Culture Collection Laboratory and confirmed by sequencing of the Internal Transcribed Spacer of rDNA. A total of 42 species were identified, of which 22 belong to the genus Aspergillus and 20 to Penicillium. Penicillium isolates showed uniform distribution from the collecting area in Tupanatinga, and the evenness indices found were 0.92 and 0.88 in Tupanatinga and Ibimirim, respectively. Among isolates of Aspergillus evenness, the value found in Tupanatinga (0.85) was very close to that found in Ibimirim (0.86). High diversity and low dominance of fungi in soil samples was observed. These results contributed to the estimation of fungal diversity in dry environments of the Caatinga, where diversity is decreasing in soils that have undergone disturbance.

https://doi.org/10.15517/rbt.v64i1.18223
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References

Blackwell, M. (2011). The Fungi: 1, 2, 3…5, 1 million species? American Journal of Botany,98, 426-238.

Bononi, V. L. R. (1998). Zigomicetos, Basidiomicetos e Deuteromicetos: noções básicas de taxonomia e aplicações biotecnológicas. São Paulo: Instituto de Botânica, Secretaria de Estado e Meio Ambiente.

Brower, J. E., & Zar, J. H. (1984). Community similarity. In J. E. Brower, & J. H. Zar (Eds.), Field & Laboratory for General Ecology (pp. 161-164). Dubuque, Iowa: W.C. Brown Publisher.

Cavalcanti, M. A. Q., Oliveira, L. G., Fernandes, M. J., & Lima, D. M. (2006). Fungos filamentosos isolados do solo em municípios na região Xingó, Brasil. Acta Botanica Brasilica, 20, 831-837.

Clark, F. E. (1965). Agar-plate method for total microbial count. In C. A. Black, D. D. Evans, J. L. White, L. E. Ensminger, F. E. Clark, & R. C. Dinaver (Eds.), Methods of soil analysis, Part 2. Chemical and microbiological properties (pp. 1460-1466). USA: American Society of Agronomy, Soil Science Society of America.

Clarke, K. R., & Warwick, R. M. (1994). Changes in Marine Communities: an Approach to Statistical Analysis and Interpretation. Bournemouth. Plymouth Marine Laboratory. Bournemouth: Bourne Press Limited.

Cruz, R., Santos, C., Lima, J. S., Moreira, K. A., & Souza-Motta, C. M. (2013). Diversity of Penicillium in soil of Caatinga and Atlantic Forest areas of Pernambuco, Brazil: an ecological approach. Nova Hedwigia, 97, 543-556.

Cullen, L. Jr., Rudran, R., & Valladares-Padua, C. (2004). Métodos de estudo em biologia da conservação e manejo da vida silvestre. Brasil: Universidade Federal do Paraná.

De Bellis, T., Kernaghan, G., & Widden, P. (2007). Plant community influences on soil microfungal assemblages in boreal mixed-wood forests. Mycologia, 99, 356-367.

De Fede, K. L., Panaccione, D. G., & Sextone, A. J. (2001). Characterization of dilution enrichment cultures obtained from size-fractionated soil bacteria by BIOLOGR community-level physiological profiles and restriction analysis of 16S rDNA genes. Soil Biology and Biochemistry, 33, 1555-1563.

Góes-Neto, A., Loguercio-Leite, C., & Guerrero, R. T. (2005). DNA Extraction from frozen field-collected and dehydrated herbarium fungal basidiomata: perform of SDS and CTAB-based methods. Biotemas, 18, 19-32.

Grayston, S. J., Grifftih, G. S., Mawdesley, J. L., Campebell, C. D., & Bardgett, R. D. (2001). Accounting of variability in soil microbial communities of temperate upland grassland ecosystem. Soil Biology and Biochemistry, 33, 533-551.

Hammer, O., Harper, D. A. T., & Ryan, P. D. (2001). PAST: Palaeontological Statistics software package for education and data analysis. Palaeontologia Electronica, 4, 1-9.

Houbraken, J., & Samson, R. A. (2011). Phylogeny of Penicillium and the segregation of Trichocomaceae into three families. Studies in Mycology, 70, 1-51.

Jurjević, Z., Peterson, S. W., Stea, G., Solfrizzo, M., Varga, J., Hubka, V., & Perrone, G. (2012). Two novel species of Aspergillus section Nigri from indoor air. IMA Fungus, 3, 159-173.

Klich, M. A. (2002). Biogeography of Aspergillus species in soil and litter. Mycologia, 94, 21-27.

Lambais, M. R., Cury, J. C., Maluche-Baretta, C. R. D., & Büll, R. C. (2005). Diversidade Microbiana nos Solos: Definindo Novos Paradigmas. In P. Vidal-Torrado, L. R. F. Alleoni, M. Cooper, A. P. da Silva, & E. J. Cardoso (Eds.), Tópicos em ciência do solo (pp. 43-84). Brasília: Sociedade Brasileira de Ciência do Solo.

Monteiro, M. C. P. (2012). Identificação de fungos dos gêneros Aspergillus e Penicillium em solos preservados do cerrado (Master Thesis). Universidade Federal de Lavra, Brasilia.

Moreira, F. M. S., & Siqueira, J. O. (2006). Microbiologia e bioquímica do solo. Lavras: Universidade Federal de Lavras.

Nielsen, U. N., Osler, G. H. R., Campbell, C. D., Burslem, D. F. R. P., & van Der Wal, R. (2010). The influence of vegetation type, soil properties and precipitation on the composition of soil mite and microbial communities at the landscape scale. Journal of Biogeography, 37, 1317-1328.

Nilsson, R. H., Kristiansson, E., Ryberg, M., Hallenberg, N., & Larsson, K. H. (2008). Intraspecific ITS variability in the kingdom fungi as expressed in the international sequence databases and its implications for molecular species identification. Evolutionary Bioinformatics 4, 193-201.

Oliveira, L. G., Cavalcanti, M. A. Q., Fernandes, M. J. S., & Lima, D. M. M. (2013). Diversity of filamentous fungi isolated from the soil in the semiarid area, Pernambuco, Brazil. Journal of Arid Environments, 95, 49-54.

Øvreås, L. (2000). Population and community level approaches for analysing microbial diversity in natural environments. Ecology Letters, 3, 236-251.

Pitt, J. I. (1991). A laboratory Guide to Common Penicillium Species. North Ryde: N.S.W: Commonwealth Scientific and Industrial Research Organization.

Pitt, J. I., & Hocking, A. D. (2009). Fungi and Food Spoilage. Australia: Springer US.

Rodal, M. J. N., Andrade, K. V. S., Sales, M. F., & Gomes, A. P. S. (1998). Fitosociologia do componente lenhoso de um refúgio vegetacional no município de Buique, Pernambuco. Revista Brasileira de Biologia, 58, 517-526.

Samson, R. A., & Frisvad, J. C. (2004). Penicillium Subgenus Penicillium: new Taxonomics Schemes, Mycotoxins and Other Extrolites. Studies in Mycology, 49, 1-260.

Samson, R. A., & Houbraken, J. (Eds.) (2011). Phylogenetic and taxonomic studies on the genera Penicillium and Talaromyces. Studies in Mycology, 7. The Netherlands: CBS-KNAW Fungal Biodiversity Centre.

Sánchez-Azofeifa, G. A., Quesada, M., Rodriguez, J. P., Nassar, J. M., Stoner, K. E., Castillo, A., Garvin, T., Zent, E. L., Calvo-Alvarado, J. C., Kalacska, M. E. R., Fajardo, L., Gamon, J. A., & Cuevas-Reyes P. (2005). Research priorities for neotropical dry forests. Biotropica, 37, 477-485.

Santos, R. M., Oliveira-Filho, A. T., Eisenlohr, P. V., Queiroz, L. P., Cardoso, D. B. O. S., & Rodal, M. J. N. (2012). Identity and relationships of the Arboreal Caatinga among other floristic units of seasonally dry tropical forests (SDTFs) of north-eastern and Central Brazil. Ecology and Evolution, 2, 409-428.

Schnittler, M., & Stephenson, S. L. (2000). Myxomycetes biodiversity in four different forest types in Costa Rica. Mycologia, 92, 626-637.

Silva, D. C. V., Tiago, P. V., Mattos, J. L. S., Paiva, L. M., & Souza-Motta, C. M. (2011). Isolamento e seleção de fungos filamentosos do solo de sistemas agroflorestais do Município de Bom Jardim (PE) com base na capacidade de produção de enzimas hidrolíticas. Revista Brasileira de Botânica, 34, 607-610.

SNE - Sociedade Nordestina de Ecologia. (2002). Projeto técnico para a criação do Parque Nacional do Catimbau/PE – versão final, em cumprimento ao contrato n º 086-00/02, subprojeto “Proposta para criação do Parque Nacional do Catimbau/PE”. Recife: Sociedade Nordestina de Ecologia (SNE).

White, T. J., Bruns, T., Lee, S., & Taylor, J. (1990). Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In M. A. Innis, D. H. Gelfand, J. J. Shinsky, & T. J. White (Eds.), PCR protocols: A guide to methods and applications (pp. 315-322). San Diego: Academic.

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