Odovtos International Journal of Dental Sciences ISSN Impreso: 1659-1046 ISSN electrónico: 2215-3411

OAI: https://www.revistas.ucr.ac.cr/index.php/Odontos/oai
Inmunoexpresión de ALDH1A1, FGFR2, CD44 y Caspasa-3 en Carcinoma Oral de Células Escamosas y Leucoplasias: un estudio piloto
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Palabras clave

Oral cancer; Squamous cell carcinoma; Leukoplakia; ALDH1A1; FGFR2; Caspase-3; CD44.
Cáncer bucal; Carcinoma oral de células escamosas; Leucoplasia; ALDH1A1; FGFR2; Caspasa-3; CD44.

Cómo citar

Boza Oreamuno, Y. V., & Reyes-Carmona, J. F. (2023). Inmunoexpresión de ALDH1A1, FGFR2, CD44 y Caspasa-3 en Carcinoma Oral de Células Escamosas y Leucoplasias: un estudio piloto. Odovtos International Journal of Dental Sciences, 25(2), 103–111. https://doi.org/10.15517/ijds.2022.50369

Resumen

La actual evidencia científica enfatiza la importancia de reconocer biomarcadores viables para el diagnóstico y tratamiento temprano del cáncer oral. Nuestro estudio piloto analizó la expresión y distribución espacial de ALDH1A1, FGFR2, caspasa-3 y CD44 en carcinoma oral de células escamosas (COCE) y en leucoplasia con o sin displasia de la mucosa oral. Las muestras incluidas en parafina de COCE (n=5), con (n=5) y sin (n=5) displasia fueron obtenidas mediante biopsias incisionales, las cuales se procesaron utilizando técnicas histoquímicas convencionales. El análisis inmunohistoquímico se realizó utilizando anticuerpos contra ALDH1A1, FGFR2, caspasa-3 y CD44. Las imágenes de las secciones de cada muestra fueron analizadas según la intensidad de inmunoexpresión de cada marcador y se clasificaron en diferentes escalas (scores). Se realizó la prueba de Kruskal-Wallis (valores de p<0,05). Nuestros resultados demostraron una diferencia estadística en la expresión de todos los inmunomarcadores entre COCE y las muestras con leucoplasia sin displasia, siendo más significativa en FGFR2 y ALDH1A1. Considerando las limitaciones de este estudio, los datos sugieren que la presencia de displasia en la mucosa oral es un importante predictor clínico-patológico de transformación maligna.

https://doi.org/10.15517/ijds.2022.50369
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