Odovtos - International Journal of Dental Sciences ISSN Impreso: 1659-1046 ISSN electrónico: 2215-3411

OAI: https://www.revistas.ucr.ac.cr/index.php/Odontos/oai
3D Printing Characteristics and Mechanical Properties of a Bio Scaffold Obtained from a Micro-CT Scan, Using the Fused Deposition Modeling Technique
PDF
HTML
EPUB

Keywords

Bio scaffolding; PLA; 3D printing; FDM; Biomaterial; Bone [AND] defect; Diatoms; Calcium phosphate.
Bioandamiaje; PLA; Impresión 3D; FDM; Biomaterial; Defecto[AND]óseo; Diatomeas; Fosfato de calcio.

How to Cite

González-Sánchez, N., Jensen-Líos, N., Hernández-Montoya, D., Campos Zumbado, J. E., & Oviedo-Quirós, J. (2023). 3D Printing Characteristics and Mechanical Properties of a Bio Scaffold Obtained from a Micro-CT Scan, Using the Fused Deposition Modeling Technique. Odovtos - International Journal of Dental Sciences, 25(2), 112–122. https://doi.org/10.15517/ijds.2022.52671

Abstract

The objective is to determine which biopolymer has the best 3D printing characteristics and mechanical properties for the manufacture of a bioscaffold, using the fused deposition printing technique, with models generated from an STL file obtained from a Micro-CT scan taken from a bovine iliac crest bone structure. Through an experimental exploratory study, three study groups of the analyzed biopolymers were carried out with thirteen printed structures of each one. The first is made of 100% PLA. The second, 90B, we added 1g of diatom extract, and the third, 88C, differs from the previous one in that it also contains 1g of calcium phosphate. The 39 printed structures underwent a visual inspection test, which required the fabrication of a gold standard scaffold in resin, with greater detail and similarity to the scanned bone structure. Finally, the structures were subjected to a compressive force (N) to obtain the modulus of elasticity (MPa) and compressive strength (MPa) of each one of them. A statistically significant difference (p=0.001) was obtained in the printing properties of the biomaterial 88C, compared to 90B and pure PLA and the 88C presented the best 3D printing characteristics. In addition, it also  presented the best mechanical properties compared to the other groups of materials. Although the difference between these was not statistically significant (p=0.388), in the structures of the 88C biomaterial, values of compressive strength (8,84692 MPa) and modulus of elasticity (43,23615 MPa) were similar to those of cancellous bone in the jaws could be observed. Because of this result, the 88C biomaterial has the potential to be used in the manufacture of bioscaffolds in tissue engineering.

https://doi.org/10.15517/ijds.2022.52671
PDF
HTML
EPUB

References

Chaudhari A.A., Vig K., Baganizi D.R., Sahu R., Dixit S., Dennis V., et al. Future prospects for scaffolding methods and biomaterials in skin tissue engineering: A review. Int J Mol Sci. 2016 Nov 25; 17 (12): 1974.

Murphy W., Black J., Hastings G. Handbook of biomaterial properties. 2nd ed. New York: Springer. 2016.

Bose S., Vahabzadeh S., Bandyopadhyay A. Bone tissue engineering using 3D printing. Mater Today (Kidlington). 2013 Dec 01; 16 (12): 496-504.

Hutmacher D.W., Schantz J.T., Lam C.X.F., Tan K.C., Lim T.C. State of the art and future directions of scaffold-based bone engineering from a biomaterials perspective. J Tissue Eng Regen Med. 2007 Jul-Aug; 1 (4): 245-260.

Amoda A., Borkiewicz L., Rivero-Müller A., Alam P. Sintered nanoporous biosilica diatom frustules as high-efficiency cell-growth and bone-mineralization platforms. Mater Today Commun. 2020 Feb 24; 1-9.

Serra T., Mateos-Timoneda M.A., Planell J.A., Navarro M. 3D printed PLA-based scaffolds: a versatile tool in regenerative medicine: A versatile tool in regenerative medicine. Organogenesis. 2013 Oct 1; 9 (4); 239-244.

Rodrigues N., Benning M., Ferreira A.M., Dixon L., Dalgarno K. Manufacture and characterization of porous PLA scaffolds. Proceeding CIRP. 2016 Aug 26; 49: 33-8.

Orhan K. Micro-computed Tomography (micro-CT) in Medicine and Engineering. 1st ed.New York: Springer. 2020.

Maher S., Kumeria T., Aw M.S., Losic D., Martín-del-Campo M., Rosales-Ibañez R., et al. Diatom Silica for Biomedical Applications: Recent Progress and Advances. Advanced Healthcare Materials. 2018 Oct; 7 (19): e1800552 .

Martín-del-Campo M., Rosales-Ibañez R., Rojo L. Biomaterials for Cleft Lip and Palate Regeneration. International Journal of Molecular Sciences. 2019 May 02; 20 (9): 2176.

Chacón J.M., Caminero M.A., García-Plaza E., Núñez P.J. Additive manufacturing of PLA structures using fused deposition modeling: Effect of process parameters on mechanical properties and their optimal selection. Mater Des. 2017 Jun 15; 124: 143-57.

Bakhtiar S.M., Butt H.A., Zeb S., Quddusi D.M., Gul S., Dilshad E. 3D Printing Technologies and Their Applications in Biomedical Science. In: Omics Technologies and Bio-Engineering. Elsevier. 2018; 167-189.

Zhang L., Yang G., Johnson B.N., Jia X. Three-dimensional (3D) printed scaffold and material selection for bone repair. Acta Biomater. 2019 Jan 15; 84: 16-33.

Gibson I., Rosen D., Mahyar K. Additive Manufacturing technologies. 3rd ed. Atlanta: Springer. 2020.

The story behind Prusament [Internet]. Prusament. 2021 [cited 26 October 2021]. Available at: https://prusament.com/es/la-historia-detras-de-prusament/

Denry I., Kuhn L.T. Design and characterization of calcium phosphate ceramic scaffolds for bone tissue engineering. Dental Materials. 2016 Jan 32; (1): 43-53.

Cardona C., Curdes A., Isaacs A. Effects of filament diameter tolerances in fused filament fabrication. IU. J. Undergrad. 2016 May 31; 2 (1): 44-47.

Diab M., Mokari T. Bioinspired Hierarchical Porous Structures for Engineering Advanced Functional Inorganic Materials. Adv Mater. 2018 Oct; 30 (41): e1706349.

Brézulier D., Chaigneau L., Jeanne S., Lebullenger R. The Challenge of 3D Bioprinting of Composite Natural Polymers PLA/Bioglass: Trends and Benefits in Cleft Palate Surgery. Biomedicines. Oct 27; 9 (11): 1553.

Displer T., Fournier N., et al. Polymer-Bioactive Glass Composite Filaments for 3D Scaffold Manufacturing by Fused Deposition Modeling: Fabrication and Characterization. Front Bioeng Biotechnol. 2020 Jun 24; 8: 55221.

Wasti S. Adhikari S. Use of Biomaterials for 3D Printing by Fused Deposition Modeling Technique: A Review. Front Chem. 2020 May 7; 8: 315.

Abdelhamid M., Pil Pack S. Biomimetic and Bioinspired Silicifications: Recent Advances for Biomaterial Design and Applications. Acta Biomater. 2021 Jan 15;120: 38-56.

Reid A., Buchanan F., et al. A review on diatom biosilicification and their adaptive ability to uptake other metals into their frustules for potential application in bone repair. J Mater Chem B. 2021 Sep 14; 9 (34): 6728-6737.

Gjerde C., Mustafa K., et al. Cell therapy induced regeneration of severely atrophied mandibular bone in a clinical trial. Stem Cell Research and Therapy. 2018 Aug 9; 9 (1): 213.

Shafiee A., Atala A. Tissue Engineering: Towards a New Era of Medicine. Reviews in advance. Annu Rev Med.2017 Jan; 68: 29-40.

Perić Kačarević Z., Rider P., et al. An Introduction to bone tissue engineering, The International Journal of Artificial Organs. 2020 Feb; 43 (2): 69-86.

Ghassemi T., Shahroodi A., Ebrahimzadeh M.H., Mousavian A., Movaffagh J., Moradi A. Current concepts in scaffolding for bone tissue engineering. Arch Bone Jt Surg. 2018 Mar; 6 (2): 90-9.

César-Juárez A., Olivos-Meza A., Landa-Solís C., Cárdenas-Soria V., Silva-Bermúdez P., Suárez-Ahedo C. et al. Use and application of 3D printing and bioprinting technology in medicine. Rev. Fac. Med. (Mex.). 2018 Dec; 61 ( 6 ): 43-51.

Comments

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

Copyright (c) 2022 CC-BY-NC-SA 4.0

Downloads

Download data is not yet available.