Revista Clínica Escuela de Medicina UCR-HSJD ISSN electrónico: 2215-2741

OAI: https://www.revistas.ucr.ac.cr/index.php/clinica/oai
First report of the NDM gene and Detection of OXA-24 in Acinetobacter baumanii at the Hospital Nacional Regional de Santa Ana.

Abstract

This investigation was carried out in one of the most important hospitals in El Salvador. With the intention of identifying the resistance genes of the most isolated bacteria in clinical samples of the Hospital based on the identification by VITEK2 and the detection of Oxacilinasa (OXA) -51 by PCR, for the moment a total of 82 samples have been processed, within the main we discovered the presence of OXA-24 in all Imipenem resistant isolates has been determined, as well as four isolates identified the presence of the NDM ((New Delhi metallo-beta-lactamase) gene.

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References

N. Woodford, M. Ellington, J. Coelho, J. Turton, M. Ward, S. Brown, S. Amyes, D. Multiplex PCR for genes encoding prevalent OXA carbapenemases in Acinetobacter spp. International Journal of Antimicrobial Agents. 2006; 27:351—353. http://dx.doi.org/10.1016/j.ijantimicag.2006.01.004

Adam, M. A., & Elhag, W. I. Prevalence of metallo-β-lactamase acquired genes among carbapenems susceptible and resistant Gram-negative clinical isolates using multiplex PCR, Khartoum hospitals, Khartoum Sudan. BMC infectious diseases. 2018; 18(1), 668. https://doi.org/10.1186/s12879-018-3581-z

Mariya Afzal-Shah, Neil Woodford, David M. Characterization of OXA-25, OXA-26, and OXA-27, Molecular Class D β-Lactamases Associated with Carbapenem Resistance in Clinical Isolates of Acinetobacter baumannii. Antimicrobial agents and chemotherapy. 2001; 45(2), 583–588. https://doi.org/10.1128/AAC.45.2.583-588.2001

M. J. Ellington, J. Kistler, D. M. Livermore, N. Multiplex PCR for rapid detection of genes encoding acquired metallo lactamases. Journal of Antimicrobial Chemotherapy. 2006; 59: 321-322. http://dx.doi.org/10.1093/jac/dkl481

Leverstein-van Hall MA, M Blok HE, T Donders AR, Paauw A, Fluit AC, Verhoef J. Multidrug Resistance among Enterobacteriaceae Is Strongly Associated with the Presence of Integrons and Is Independent of Species or Isolate Origin. The Journal of Infectious Diseases. 2003;187(2):251-259. doi:10.1086/345880

Patrice Nordmann, Laurent Poirel, Timothy R. Walsh, David M. Livermore. The emerging NDM carbapenemases. Trends in Microbiology. 2011; 19: 588-595. http://dx.doi.org/10.1016/j.tim.2011.09.005

Mudathir Abdallah Adam, Wafa I. Prevalence of metallo-β-lactamase acquired genes among carbapenems susceptible and resistant Gram-negative clinical isolates using multiplex PCR, Khartoum hospitals, Khartoum Sudan. BMC infectious diseases. 2018. 18(1), 668. https://doi.org/10.1186/s12879-018-3581-z

N. Petrosillo, M. F. Proietti, L. Cecchini, M. Masala, C. Franchi, M. Venditti, S. Esposito, E. Nicastri, P. Chinello. Combined colistin and rifampicin therapy for carbapenem-resistant Acinetobacter baumannii infections: clinical outcome and adverse events. Clinical Microbiology and Infection. 2005; 11: 682-683. http://dx.doi.org/10.1111/j.1469-0691.2005.01198.x

Sobieszczyk ME. Combination therapy with polymyxin B for the treatment of multidrug-resistant Gram-negative respiratory tract infections. Journal of Antimicrobial Chemotherapy. 2004;54(2):566-569. doi:10.1093/jac/dkh369

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Copyright (c) 2023 Adan Alexis Acosta

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