Abstract
The main challenge in root canal treatment is to reduce bacterial load good enough to ensure success and to avoid reinfection. However, the evidence of biofilm along the root canal system and the inability of current instruments and irrigants to eliminate bacterial biofilm have built a barrier toward a higher favorable prognosis of the root canal treatment, which has not change in the past five decades. Regarding this scenery, research in endodontics have been addressed to find new strategies or protocols to fight the war against biofilm. From conventional irrigants solutions to nanotechnology products are been tested, several in vitro and clinical studies are performance every day in this incessant search. So, what is next, where are we going in endodotics biofilm?
References
Ramachandran Nair P. N., Harndt E., Winkler K., et al. Light and electron microscopic studies of root canal flora and periapical lesions. J. Endod. Elsevier; 1987 Jan; 13 (1): 29–39.
Molven O., Olsen I., Kerekes K. Scanning electron microscopy of bacteria in the apical part of root canals in permanent teeth with periapical lesions. Endod Dent Traumatol. 1991 Oct; 7 (5):.226–9.
Ricucci D., Siqueira J. F., Lopes WSP, Vieira AR, Rôças I. N. Extraradicular infection as the cause of persistent symptoms: a case series. J Endod. 2015 Feb; 41 (2): 265–73.
Siqueira J. F., Rôças I. N., Lopes H. P. Patterns of microbial colonization in primary root canal infections. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2002 Feb; 93 (2): 174–8.
Sundqvist G. Ecology of the root canal flora. J. Endod. 1992 Sep ;18(9): 427–30.
Möller A. J. Microbiological examination of root canals and periapical tissues of human teeth. Methodological studies. Odontol Tidskr. 1966 Dec 20 74 (5): Suppl:1-380.
Bergenholtz G. Micro-organisms from necrotic pulp of traumatized teeth. Odontol Revy. 1974. 25 (4): 347–58.
Costerton J. W., Cheng K. J., Geesey G. G., Ladd T. I., Nickel J. C., Dasgupta M., et al. Bacterial biofilms in nature and disease. Annu Rev Microbiol [Internet]. 1987 41:435–64.
Voběrková S., Hermanová S., Hrubanová K., Krzyžánek V. Biofilm formation and extracellular polymeric substances (EPS) production by Bacillus subtilis depending on nutritional conditions in the presence of polyester film. Folia Microbiol (Praha). 2015.
Flemming H-C, Wingender J. The biofilm matrix. Nat Rev Microbiol. 8 (9): 623–33. Available from: http://www.ncbi.nlm.nih.gov/pubmed/20676145
Siqueira J. F., Rôças I. N., Ricucci D. Biofilms in endodontic infection. Endod Top. 2010 Mar. 22 (1): 33–49.
Marsh P. D. Dental plaque as a microbial biofilm. Caries Res. 38 (3): 204–11.
Lima K. C., Coelho L. T., Pinheiro I.V.A., Rôças I. N., Siqueira J. F. Microbiota of dentinal caries as assessed by reverse-capture checkerboard analysis. Caries Res. 2011; 45 (1): 21–30.
Munson M. A., Banerjee A., Watson T. F., Wade W. G. Molecular analysis of the microflora associated with dental caries. J. Clin Microbiol. American Society for Microbiology (ASM); 2004 Jul; 42 (7): 3023–9.
Costerton W., Veeh R., Shirtliff M., Pasmore M., Post C., Ehrlich G. The application of biofilm science to the study and control of chronic bacterial infections. J. Clin Invest. 2003 Nov; 112 (10): 1466–77.
Nair P. N. R., Henry S., Cano V., Vera J. Microbial status of apical root canal system of human mandibular first molars with primary apical periodontitis after one-visit; endodontic treatment. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2005 Feb; 99 (2): 231–52.
Ricucci D., Siqueira J. F. Apical actinomycosis as a continuum of intraradicular and extraradicular infection: case report and critical review on its involvement with treatment failure. J. Endod. 2008 Sep; 34 (9): 1124–9.
Tronstad L., Sunde P. T. The evolving new understanding of endodontic infections. Endod Top. Munksgaard International Publishers; 2003 Nov; 6 (1): 57–77.
Svensater G., Bergenholtz G. Biofilms in endodontic infections. Endod Top. Munksgaard International Publishers; 2004 Nov.
Parsek M. R., Singh P. K. Bacterial biofilms: an emerging link to disease pathogenesis. Annu Rev Microbiol. 2003. 57: 677–701.
Hall-Stoodley L., Stoodley P. Evolving concepts in biofilm infections. Cell Microbiol. 2009; 11 (7): 1034–43.
Ricucci D., Siqueira J. F. Biofilms and apical periodontitis: study of prevalence and association with clinical and histopathologic findings. J. Endod. 2010 Aug 36 (8): 1277–88.
Nair P. N. R., Henry S., Cano V., Vera J. Microbial status of apical root canal system of human mandibular first molars with primary apical periodontitis after one-visit; endodontic treatment. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2005 Feb; 99 (2): 231–52.
Friedman S., Abitbol S., Lawrence H. P. Treatment outcome in endodontics: the Toronto Study. Phase 1: initial treatment. J. Endod. 2003 Dec;29 (12): 787–93.
Kishen A., Sum C-P, Mathew S., Lim C-T. Influence of irrigation regimens on the adherence of Enterococcus faecalis to root canal dentin. J. Endod. 2008 Jul 2016 Sep 20]; 34 (7): 850–4.
Kostakioti M., Hadjifrangiskou M., Hultgren S. J. Bacterial biofilms: development, dispersal, and therapeutic strategies in the dawn of the postantibiotic era. Cold Spring Harb Perspect Med. 2013 Apr; 3 (4).
Jansen B., Kohnen W. Prevention of biofilm formation by polymer modification. J. Ind Microbiol. 1995 Oct 15 (4): 391–6.
Balaban N., Giacometti A., Cirioni O., Gov Y., Ghiselli R., Mocchegiani F., et al. Use of the Quorum-Sensing Inhibitor RNAIII- Inhibiting Peptide to Prevent Biofilm Formation In Vivo by Drug-Resistant Staphylococcus epidermidis. 2003;187.
George S., Kishen A. Photophysical, photochemical, and photobiological characterization of methylene blue formulations for light-activated root canal disinfection. J. Biomed Opt. 12 (3): 34029.
Shrestha A., Kishen A. Antibacterial Nanoparticles in Endodontics: A Review. J. Endod. 2016;42(10):1417–26.