@article{Velasco-Blanco_Álvarez González_Abdo de la Parra_Rodríguez-Ibarra_Ibarra-Castro_Maytorena-Verdugo_Arias-Jiménez_Peña Marín_2023, title={ Ontogeny of digestive enzymes in larvae of the Clown Anemonefish, Amphiprion ocellaris (Perciformes: Pomacentridae)}, volume={71}, url={https://www.revistas.ucr.ac.cr/index.php/rbt/article/view/51085}, DOI={10.15517/rev.biol.trop..v71i1.51085}, abstractNote={<p><strong>Introduction:</strong> Clown anemonefish (<em>Amphiprion ocellaris</em>) is the most popular fish species in the marine aquarium trade, however, there is a lack of information on their digestive physiology during larvae ontogeny, valuable information that helps in the design of specific diets, as well as management protocols for the species. <strong>Objective:</strong> Characterize the early development of digestive enzymes of <em>A. ocellaris</em> during larvae ontogeny. <strong>Methods:</strong> From hatching until the 38<sup>th</sup> day after hatching (DAH), the specific activity of acid and alkaline proteases, trypsin, chymotrypsin, leucine aminopeptidase and lipase were analyzed, while acid and alkaline proteases zymograms were performed. <strong>Results:</strong> At hatching, all measured enzymes activities were detected. Acid proteases increased in activity from hatching until the 38<sup>th</sup> DAH. Alkaline proteases, trypsin, chymotrypsin, and leucine aminopeptidase showed the same pattern and maximum activity on the 8<sup>th</sup> DAH, decreasing at the 38<sup>th</sup> DAH. Lipase activity showed peaks on the 8<sup>th</sup> and 30<sup>th</sup> DAH. Acid zymogram showed a single band, appearing on the 8<sup>th</sup> DAH. A total of eight alkaline proteases were revealed (154.2, 128.1, 104.0, 59.8, 53.5, 41.9, 36.5 and 25.1 KDa), showing seven bands on the 1<sup>st</sup> DAH and all bands from the 3<sup>rd</sup> to 8<sup>th</sup> DAH, decreasing at two bands (41.9 and 25.1 KDa) in the 38<sup>th</sup> DAH. <strong>Conclusion:</strong> <em>A. ocellaris</em> shows a functional stomach on the 8<sup>th</sup> DAH, where the species on the 38<sup>th</sup> DAH shows digestive enzymatic pattern to omnivore with a tendency to carnivory.</p> <p><strong>Objective:</strong> Characterize the early development of digesive enzymes of <em>A. ocellaris</em> during larval ontogeny.</p> <p><strong>Methods:</strong> From hatching until the 38<sup>th</sup> days after hatching (DAH), the specific activity of acid and alkaline proteases, trypsin, chymotrypsin, leucine aminopeptidase and lipase were analysed, while acid and alkaline proteases zymograms were perfomed.</p> <p><strong>Results:</strong> At hatching, all measured enzymes activities were detected. Acid proteases increased in activity from hatching until the 38<sup>th</sup> DAH. Alkaline proteases, trypsin, chymotrypsin and leucine aminopeptidase showed same pattern and maximum activity on the 8<sup>th</sup> DAH, decreasing at the 38<sup>th</sup> DAH. Lipase showed activity peaks on the 8<sup>th</sup> and 30<sup>th </sup>DAH. Acid zymogram showed a single band, appearing on the 8<sup>th</sup> DAH. Total of eight alkaline proteases were reveled (154.2, 128.1, 104.0, 59.8, 53.5, 41.9, 36.5 and 25.1 KDa), showing seven bands on the 1<sup>st</sup> DAH and all band from the 3<sup>rd</sup> to 8<sup>th</sup> DAH, decreasing at two bands (41.9 and 25.1 KDa) in the 38<sup>th</sup> DAH.</p> <p><strong>Conclusion:</strong> Therefore, <em>A. ocellaris</em> shows functional stomach on the 8<sup>th</sup> DAH, where the species on the 38<sup>th</sup> DAH shows digestive enzymatic pattern to omnivore with a tendency to carnivory.</p>}, number={1}, journal={Revista de Biología Tropical}, author={Velasco-Blanco, Gabriela and Álvarez González, Carlos Alfonso and Abdo de la Parra, Maria Isabel and Rodríguez-Ibarra, Luz Estela and Ibarra-Castro, Leonardo and Maytorena-Verdugo, Claudia I. and Arias-Jiménez, José Natividad and Peña Marín, Emyr Saul}, year={2023}, month={Mar.}, pages={e51085} }