TY - JOUR AU - Avila-Díaz, Irene AU - Sarabia-Ochoa, Marcela Esmeralda AU - Carlos-Gómez, Alonso AU - Salgado-Garciglia, Rafael PY - 2010/04/26 Y2 - 2024/03/28 TI - Callus growth and plant regeneration in Laelia speciosa (Orchidaceae) JF - Lankesteriana: International Journal on Orchidology JA - LIJO VL - 10 IS - 1 SE - Articles DO - 10.15517/lank.v10i1.18319 UR - https://www.revistas.ucr.ac.cr/index.php/lankesteriana/article/view/18319 SP - AB - <div class="page" title="Page 1"><div class="section"><div class="layoutArea"><div class="column"><p><span>Laelia speciosa </span><span>is an endangered epiphytic orchid, endemic to México. It is thought that the asexual reproduction </span><span>in vitro </span><span>could be one of the actions to counteract the massive extraction of individuals from their natural populations. The growth and differentiation of callus tissues derived from leaf explants of </span><span>L. speciosa </span><span>were investigated in Murashige and Skoog medium (MS) with 30 g l</span><span>-1 </span><span>sucrose and five concentrations (0.0, </span><span>0.25, 0.5, 1.0, and 2.5 g l</span><span>-1</span><span>) of naphthaleneacetic acid (NAA) with benzyladenine (BA) (0.0, 0.25, 0.5, 1.0, and 2.5 g l</span><span>-1</span><span>). Leaf explants from </span><span>in vitro </span><span>plantlets formed callus tissue on MS medium supplemented with 2.5 mg l</span><span>-1 </span><span>BA while mature leaves did not respond. Diameter of the callus tissues averaged 1.25 cm after eight weeks of culture. PLBs development was achieved on MS medium supplemented with 2.5 mg l</span><span>-1 </span><span>NAA and 1 mg l</span><span>-1 </span><span>BA. The formation of plantlets was successfully obtained in MS supplemented with 0.5 mg l</span><span>-1 </span><span>of NAA and 0.1 mg l</span><span>-1 </span><span>of gibberellic acid (GA</span><span>3</span><span>). Obtained plantlets were successfully acclimatized in a greenhouse with a survival rate of 70%. </span></p></div></div></div></div> ER -