TY - JOUR AU - Wilson, Mark AU - Jost, Lou PY - 2011/11/20 Y2 - 2024/03/29 TI - Phylogenetic analysis of the Andean genus Brachycladium Luer (syn. Oreophilus Higgins & Archila) and closely related genera based on nuclear ITS sequencing JF - Lankesteriana: International Journal on Orchidology JA - LIJO VL - 11 IS - 3 SE - DO - 10.15517/lank.v11i3.18305 UR - https://www.revistas.ucr.ac.cr/index.php/lankesteriana/article/view/18305 SP - AB - <div class="page" title="Page 1"><div class="layoutArea"><div class="column"><p><span>We previously reported that nrITS sequencing and phylogenetic analysis revealed at least four cryptic species within </span><span>Brachycladium nummularium </span><span>(syn. </span><span>Oreophilus nummularius</span><span>). Sequencing of additional species in the genus revealed two major clades within </span><span>Brachycladium</span><span>, the “nummularium”-complex plus </span><span>B. stalactites </span><span>and Luer’s section </span><span>Amplectentes</span><span>. Since the relationship of </span><span>Brachycladium </span><span>to </span><span>Andinia </span><span>had been noted earlier, a number of </span><span>Andinia </span><span>species were also included. Two clades were observed, a clade containing </span><span>A. lappacea, A. pensilis, </span><span>and </span><span>A. vestigipetala </span><span>that was closely related to </span><span>Brachycladium </span><span>and a second, more distantly related clade containing </span><span>A. dalstroemii, A. pogonion</span><span>, and </span><span>A. schizopogon</span><span>. </span>Samples of two additional genera, Masdevalliantha Szlachetko &amp; Margonska and Xenosia Luer, were included in the analysis based upon morphological similarity to Andinia. These two genera formed a clade that was sister to both Brachycladium and Andinia. Only the node subtending clades of all four genera had a high level of bootstrap support, whereas nodes subtending clades of Brachycladium plus Andinia, or Brachycladium alone, had much lower support. If future chloroplast sequencing produces phylogenies congruent with nrITS, this would indicate that Andinia, Brachycladium (syn. Oreophilus), Masdevalliantha, and Xenosia should all be placed in the same genus. </p></div></div></div> ER -